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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Mouse Cardiac Pde1C Is a Direct Transcriptional Target of Pparα
doi: 10.3390/ijms19123704
Figure Lengend Snippet: Chromatin immunoprecipitation assay of H9c2 cells and WT mouse heart genomic DNA revealed that PPARα binds to two sites in the 5kb promoter region of Pde1C gene. ( A ) Schematic representation of 5kb Pde1C promoter region and locations of putative PPRE on the promoter. PPRE2 is a palindrome sequence. The arrows show plus and minus strands of DNA, respectively. ( B ) Specific locations and sequences of putative PPREs on Pde1C promoter as analyzed by Genomatix MatInspector software. ( C ) Total lysates from H9c2 cells and mouse heart treated with Wy-14,643 were analyzed by ChIP using PPARα antibody. PPARα bound to PPRE2 and PPRE3 in both H9c2 cells and WT mouse hearts. + and – refer to plus and minus strands of DNA, respectively. ( D ) ChIP of mouse heart was followed by RT-qPCR to quantify the extent of PPARα binding to Pde1C promoter. PPRE2 and PPRE3 showed 10- and 2-fold increased binding, respectively, as compared to mock. The input panel shown in C belongs to that of heart samples. The input data of H9c2 samples not shown here are similar to that of heart samples.
Article Snippet: To analyze Pde1C gene promoter, 5 kb upstream of mRNA start site was analyzed using Genomatix
Techniques: Chromatin Immunoprecipitation, Sequencing, Software, Quantitative RT-PCR, Binding Assay